The trail starts from the monastery of Agioi Pateres. After walking on the asphalt paved road for about 200 m, we reach the entrance to the footpath, where there is an informational sign.
A few meters further on, the easily discernible footpath gives way to the old stone-paved road, which is well preserved and relatively wide; it is supported on equally well preserved dry stonework.
For practically the whole way the stone-paved road and the stone steps moderating the steep roadside margin are in good repair and easy to negotiate. The route snakes its way down to the Nea Moni monastery.
Along almost the entire route, we look down on the building complex of Nea Moni. Mt. Penthodos and the Monastery of Agios Markos that stands on its summit are facing us. The view of the town of Chios and the coast of Asia Minor is breathtaking.
At higher elevations along the route the ground is rocky with sparse vegetation and the remains of burnt pine trees. In many places the natural regeneration of the forest is apparent.
The lower we descend, the thicker the vegetation. At the sides of the footpath we see strawberry trees, wild olive trees, wild grapevines, oregano, thorny burnet, and many naturally regenerated clumps of young Calabrian pines.
Close to Nea Moni the landscape changes and we see olive groves and tall cypress trees.
Before we come to the historic monastery, which also marks the end of the trail, we pass the chapel of Agios Loukas, which is the cemetery church for Nea Moni.

From Olympoi we follow the signs to the Anessomata locale, and cross the plain, where cultivated mastic, olive and fig trees predominate. We climb up the slope and in a few minutes we have reached the highest point of the trail. Here we encounter the chapel of Agios Antonios, where the ground is flat and there are a number of cypress trees and a picnic and rest area with an astounding view. The footpath continues downhill. We walk along the bank of the stream, among pines, kerm oaks, strawberry trees, oaks and mastic trees. We have a panoramic view of the village of Mesta before us, as we pass through fields with old dry stone walls that form step terraces. As we descend, we come to the old aqueduct with the stone wells and a spring. The old, well-preserved stone-paved path leads to Mesta.